The two became trapped in the room as the huge yellow and black insects covered the house.
They were able to call the fire service before making a desperate escape through an upstairs window where firefighters had propped up a ladder.
The swarm were so terrifying that firefighters said they could not even enter the home in Lower Cookham Road in Maidenhead, Berks.
Crew manager Martyn Sadler said: "I've never been called to something like this before.
"All we could see were the hornets...and they were quite large.
"The noise made by the hornets was quite substantial and we definitely made the decision not to go up there."
Firefighters arrived at the house at around 11.30pm last night and helped the pair escape the infestation.
The woman was said to have been in the bathroom when the weight of the nest forced it through the ceiling and the deadly hornets began buzzing around.
A Berkshire Fire and Rescue Service spokesman said: "The weight of the nest brought it through the property's second-floor ceiling, filling the upstairs with angry hornets and trapping the pair in their bedroom.
"Firefighters used a ladder to rescue the couple from their room.
"A pest control company was due to visit the home today, and the couple are staying with friends."
The hornets are thought to be relatives of the giant killer Asian hornets that spread to the UK in their swarms over the summer.
Reports have shown the hornets have killed at least six people since spreading to Europe.
They can grow up to 5cm long with 6mm stingers and are twice as big as our native hornets.
It is believed they eat common garden bees as well as other insects.
Pest controller Elliot Lowe told the Daily Star: “Like the European hornet, the Asian hornet is not generally aggressive.
“But they shouldn’t be provoked as stings can be painful and obviously there are reports that people have died as a result of being stung.
"It is a highly effective predator of insects, including honey bees and other environmentally beneficial insects, and can inflict significant losses to bee colonies.”
